Composite

Part:BBa_K1547001:Design

Designed by: victor yuan   Group: iGEM14_Concordia   (2014-10-08)

PSAD GFP-NLS cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1570
    Illegal AgeI site found at 916
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1703


Design Notes

40 bp Linkers surround each part. i.e. cassette builds as follows:

Linker 1 - PSAD Promoter - Linker 2 - GFP - NLS - Linker 3 - PSAD Terminator - Linker 4

These linkers provide a convenient way of amplifying each of our parts which we have submitted. All part types (promoters, genes, terminators) are surrounded by the same linkers. For example, a promoter is always flanked by linker 1 upstream, and linker 2 downstream. The sequence is also provided.



Source

Each part is amplified from a different plasmid. Plasmids were supplied by chlammycollection.org

References